Ultrastructural cytochemistry and immunocytochemistry of proteoglycans associated with epiphyseal cartilage calcification.
نویسندگان
چکیده
منابع مشابه
Light microscopic autoradiography and ultrastructural cytochemistry of sulfated proteoglycans in degenerated chondrocytes of rat epiphyseal cartilage.
Degenerating chondrocytes are often localized in the lower hypertrophic and calcifying zone of rat epiphyseal cartilage. The ability of these degenerating chondrocytes to synthesize and secrete sulfated proteoglycans was investigated using the light microscopic radiosulfate labeling method and the high-iron diamine-thiocarbohydrazide-silver proteinate (HID-TCH-SP) method for sulfated complex ca...
متن کاملVesicles Associated with Calcification in the Matrix of Epiphyseal Cartilage
Vesicles have been identified within the cartilage matrix of the upper tibial epiphyseal plate of normal mice . They were seen at all levels within the plate and usually did not appear to be in contact with cartilage cells . Vesicles were concentrated within the matrix of the longitudinal septa from the proliferative zone downward . They varied considerably in size (-300 A to --I μ) and in shap...
متن کاملVesicles Associated with Calcification in the Matrix of Epiphyseal Cartilage
Vesicles have been identified within the cartilage matrix of the upper tibial epiphyseal plate of normal mice. They were seen at all levels within the plate and usually did not appear to be in contact with cartilage cells. Vesicles were concentrated within the matrix of the longitudinal septa from the proliferative zone downward. They varied considerably in size ( approximately 300 A to approxi...
متن کاملHistology of epiphyseal cartilage calcification and endochondral ossification.
Cartilage calcification is carried out by chondrocytes as they hypertrophy and begin to secrete matrix vesicles. Calcification initiates when calcium phosphates appear inside these matrix vesicles, forming hydroxyapatite crystals that eventually break through the membrane to form calcifying globules, as in bone calcification. However, the extracellular environment in cartilage is different from...
متن کاملUltrastructural Cytochemistry
Selective extraction of specific cell components by enzyme or acid hydrolysis is possible from ultrathin sections for electron microscopy and parallel 2 micro sections for light microscopy of tissues fixed in formalin and embedded in a water-soluble polyepoxide, product X133/2097. Normal rat tissues fixed 15 minutes in formalin at 3 degrees C are more rapidly digested by proteinases than those ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Histochemistry & Cytochemistry
سال: 1983
ISSN: 0022-1554,1551-5044
DOI: 10.1177/31.9.6136541